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cdaR [2017-8-11 16:0:3]
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cdaR [2017-8-11 16:0:3]

effector protein controlling CdaA diadenylate cyclase activity
Locus
BSU01760
Isoelectric point
4.72
Molecular weight
52.78 kDa
Protein length
483 aa Sequence Blast
Gene length
1449 bp Sequence Blast
Function
regulation of c-di-AMP synthesis
Product
effector protein controlling CdaA diadenylate cyclase activity
Essential
no
Synonyms
ybbR

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
197,027 → 198,478

The protein

Catalyzed reaction/ biological activity

  • stimulates CdaA activity upon protein-protein interaction PubMed
  • Structure

  • 2KPU (one YbbR domain, from Desulfitobacterium hafniense) PubMed
  • Localization

  • cell membrane, oriented towards the outside PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Biological materials

    Mutant

  • GP999 (cdaR::cat), available in Jörg Stülke's lab
  • GP985 (cdaA-cdaR::cat), available in Jörg Stülke's lab
  • Expression vector

  • IPTG inducible expression of Strep-cdaR in E. coli: pGP2565 (in pGP172), available in Jörg Stülke's lab
  • IPTG inducible expression of His-cdaR in E. coli: pGP2557 (in pET19b), available in Jörg Stülke's lab
  • GP2031: cdaR-Strep aphA3, chromosomal expression in B. subtilis, for SPINE, available in Jörg Stülke's lab
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992.
  • Antibody

  • available in Jörg Stülke's lab
  • References

    Reviews

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    Original publications

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